Controlling Endosomal Escape Using pH-Responsive Nanoparticles with Tunable Disassembly

N Kongkatigumjorn; SA Smith; M Chen; K Fang; S Yang; ER Gillies; APR Johnston; GK Such

Journal article

Controlling Endosomal Escape Using pH-Responsive Nanoparticles with Tunable Disassembly

N Kongkatigumjorn, SA Smith, M Chen, K Fang, S Yang, ER Gillies, APR Johnston, GK Such

ACS Applied Nano Materials | AMER CHEMICAL SOC | Published : 2018

DOI: 10.1021/acsanm.8b00338

Abstract

Endosomal escape is a bottleneck in the efficient delivery of therapeutics using nanoparticles; therefore understanding how this property can be optimized is important for achieving better therapeutic outcomes. It has been demonstrated that pH-responsive nanoparticles (pHlexi nanoparticles) have potential to achieve effective escape from the endosomal compartments of the cell. In this paper a library of five pHlexi particles with tunable disassembly pH were synthesized by combining poly(ethylene glycol)-b-poly(2-(diethylamino)ethyl methacrylate) (PEG-b-PDEAEMA) with random copolymers of 2-(diethylamino)ethyl methacrylate and 2-(diisopropylamino)ethyl methacrylate. A series of cellular studie..

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University of Melbourne Researchers

Georgina Such Author

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Grants

Funding Acknowledgements

This work was supported by the Australian Research Council through the Future Fellowship Scheme (FT120100564, G.K.S.; and FT110100265, A.P.R.J.) and Centre of Excellence in Convergent Bio-Nano Science and Technology (A.P.RJ.). The authors thank Dr. E. Hanssen (Melbourne Advanced Microscopy Facility, Bio21 Molecular Science and Biotechnology Institute) for cryo-EM imaging.